Antimicrobial susceptibility in Cuban isolates of Vibrio cholerae O1 obtained from clinical samples

[摘要] Introduction: re-emergence of cholera in Haiti created a new reservoir for the increase of the seventh pandemic. This resulted in its spread to the Dominican Republic and other Caribbean countries, such as Cuba and Mexico.Objectives: study the antimicrobial susceptibility of isolates of Vibrio cholerae O1, Ogawa serotype, El Tor biotype, obtained from patients during the cholera epidemiological event occurring in Cuba from June 2012 to August 2013.Methods: a study was conducted of 144 V. cholerae isolates using the Bauer-Kirby method to determine in vitro susceptibility to nine antimicrobials: ampicillin, sulfonamide, trimethoprim/sulfamethoxazole, chloramphenicol, tetracycline, doxycycline, azithromycin, ciprofloxacin and gentamicin, in compliance with standards from the U.S. Clinical and Laboratory Standards Institute.Results: all isolates were resistant to trimethoprim/sulfamethoxazole; 98.7 % to sulfonamide and 90.3 % to ampicillin. Intermediate sensitivity values were obtained for ciprofloxacin (30.6 %) and chloramphenicol (27.1 %). Sensitivity levels above 92 % were found for first-line antimicrobials (doxycycline, tetracycline and azithromycin), as well as gentamicin. Multi-drug resistant strains were not found.Conclusions: results reveal the effectiveness in vitro of the antimicrobials used in Cuba to treat acute diarrheal disease caused by V. cholerae.

[学科分类] 传染病学

[关键词] Vibrio cholerae O1;antimicrobial susceptibility;antimicrobial resistance;surveillance