Development of a Loop-Mediated Isothermal Amplification Method for DetectingStreptococcus equi subsp. zooepidemicus and Analysisof Its Use with Three Simple Methods of Extracting DNA from Equine Respiratory TractSpecimens

[摘要] References(26)Cited-By(6)Streptococcus equi subsp. zooepidemicus(S. zooepidemicus) is a dominant pathogenic bacterium in equinepneumonia. We developed a specific loop-mediated isothermal amplification (LAMP) method,which targets the gene encoding sorbitol-6-phosphate 2-dehydrogenase(sorD), for detecting S. zooepidemicus and examinedthe clinical efficacies of its use in combination with each of 3 DNA extraction methodseasily used by veterinary practitioners, namely the Loopamp PURE DNA Extraction Kit,InstaGene Matrix and a conventional boiling method. The LAMP method plus the Loopamp PUREDNA Extraction Kit gave higher rates of positivity than the other combinations in bothclinical and spiked samples containing clinically significant concentrations (>1 ×104 CFU/ml) of S. zooepidemicus.

[学科分类] 兽医学

[关键词] DNA extraction method;equine;LAMP method;Loopamp PURE DNA Extraction Kit;Streptococcus equi subsp. zooepidemicus