Screening for Intestinal Microflora Influencing Superoxide Dismutase Activityin Mouse Cecal Mucosa

[摘要] References(18)We have suggested that intestinal microflora reduces the activity of theantioxidant enzyme superoxide dismutase (SOD) in the mouse cecal mucosa. In this study,gnotobiotic mice were used to examine the species of intestinal microflora influencing SODactivity in the cecal mucosa. The total SOD activity in the cecal mucosa of each germ-free(GF), gnotobiotic mouse with Escherichia coli,Lactobacillus and Bacteroides was significantly higherthan that in the cecal mucosa of gnotobiotic mice with chloroform-treated feces (CHF),conventionalized (CVz) mice and conventional (CV) mice (P<0.05). Inaddition, CuZnSOD mRNA expression showed similar tendencies. Our results suggest that theantioxidant defense status in the cecal mucosa is influenced by CHF inoculation.

[学科分类] 兽医学

[关键词] cecal mucosa;chloroform-treated feces;germ-free;superoxide dismutase