Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate forTrypanosoma congolense

[摘要] References(28)Cited-By(2)Animal African trypanosomosis (AAT), caused by Trypanosomacongolense, is widespread throughout sub-Saharan Africa. There are significantconcerns related to the current drugs available for the treatment of AAT due to theirlimited effectiveness across species and their adverse effects. Moreover, drug resistanttrypanosomes have recently been reported in the field. High throughput screening (HTS) oflarge chemical compound library collections is a promising approach for identifying noveldrug candidates. While HTS for Trypanozoon trypanosomes, T.brucei sspp. and T. evansi is well established, no assays havebeen developed for T. congolense. In the present study, the authorsdeveloped an ATP-based luciferase viability assay for T. congolense in a96-well plate format. The calculated 50% inhibitory concentration (IC50) valuesfor pentamidine and diminazene were 10�?100 times higher in T. congolensethan in T. brucei. This result suggests that the transporters for the 2tested compounds differ between T. congolense and T.brucei. This assay could further be applied to screen novel chemical compoundsfor the treatment of AAT caused by T. congolense.

[学科分类] 兽医学

[关键词] Animal African trypanosomosis;drug screening;luciferase assay;Trypanosoma congolense