High Genetic Diversity of Anaplasma marginale Detected fromPhilippine Cattle

[摘要] References(34)Cited-By(4)A total of 658 cattle in 6 provinces in the Philippines were screened forAnaplasma marginale infection by using a diagnostic heat-shock operon(groEL) gene-PCR assay. The screening-positive samples were furthertested using the major surface antigen protein 1a (Msp1a) gene-PCR assay.Screening PCR results showed 130 cattle (19.8%) were positive for the A.marginale infection. Subsequent amplification using the Msp1agene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeatstructures, including 20 novel structures, and 41 distinct genotypes were identified.Interestingly, multiple infections of 4 different genotypes were also observed inA. marginale-infected cattle. The present study demonstrated theprevalence and characterization of diverse genotypes of A. marginale inthe Philippine cattle.

[学科分类] 兽医学

[关键词] Anaplasma marginale;cattle;groEL;Msp1a;Philippines