Corneal-Protective Effects of an Artificial Tear Containing SodiumHyaluronate and Castor Oil on a Porcine Short-Term Dry Eye Model

[摘要] References(23)The corneal-protective effectsof an artificial tear containing sodium hyaluronate (SH) and castor oil (CO) wereevaluated on a porcine short-term dry eye model. Fresh porcine eyes with an intact corneawere treated with an artificial tear of saline, SH solution (0.1%, 0.5% or 1%), COsolution (0.5%, 1% or 5%) or a mixture solution containing 0.5% SH and 1% CO and thendesiccated for 60, 90 or 180 min. To assess corneal damage, the eyes were stained withmethylene blue (MB) or lissamine green (LG). The staining score of MB, absorbance of MBextracted from the cornea and staining density of LG increased significantly withincreasing desiccation time in untreated and all artificial tear-treated eyes, althoughthere were no significant differences in staining scores and absorbance of MB between eyestreated continuously with saline and 1% SH-treated ones at 60 and 90 min of desiccation orthe mixture-treated eyes at 60 min of desiccation. No significant differences in thestaining density of LG were also found between continuous saline-treated eyes and onesdesiccated for 60 min and treated with 1% SH and the mixture. Mild cytoplasmicvacuolations were histopathologically observed in the basal and wing cells in eyesdesiccated for 60 min and treated with 1% SH and the mixture. The mixture solutioncontaining 0.5% SH and 1% CO has protective effects against corneal desiccation similar tothose of 1% SH and would be helpful as an artificial tear.

[学科分类] 兽医学

[关键词] artificial tear;castor oil;porcine short-term dry eye model;sodium hyaluronate