Comparison of Surgical Methods of Transient Middle Cerebral Artery Occlusionbetween Rats and Mice

[摘要] References(28)Cited-By(2)Rodent models of focalcerebral ischemia that do not require craniotomy have been developed by intraluminalsuture middle cerebral artery occlusion (MCAo). Mouse MCAo models have been widely usedand extended to genetic studies of cell death or recovery mechanisms. Therefore, wecompared surgery-related parameters and techniques between such rats and mice. In rodentMCAo models, has to be considered body temperature during the operative period, as well asthe need for the use of a standardized tip in terms of the outer diameter of probes.Induction of focal cerebral ischemia was measured by neurological dysfunction parameters.Our methods could induce stable moderate-severity ischemic brain injury models andhistological alteration at 24 hr after MCAo surgery. Moreover approximately 80% (rats) and85% (mice) survival ratios were shown indicating with model engineering success. Finally,we described and compared major parameters between rats and mice, including probe size,thread insert length, operation and occlusion periods, and differences in theprocedures.

[学科分类] 兽医学

[关键词] focal cerebral ischemia;microsurgical procedure;rodent MCAo model