Canine Amino Acid Transport System Xc�?: cDNA Sequence,Distribution and Cystine Transport Activity in Lens Epithelial Cells

[摘要] References(37)Cited-By(1)The cystine transport activity of a lens epithelial cell line originated from acanine mature cataract was investigated. The distinct cystine transport activity wasobserved, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequencesof canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predictedamino acid sequences were 527 and 533 amino acid polypeptides, respectively. The aminoacid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those ofhumans. The expression of xCT in lens epithelial cell line was confirmed by western blotanalysis. RT-PCR analysis revealed high level expression only in the brain, and it wasbelow the detectable level in other tissues.

[学科分类] 兽医学

[关键词] cysteine/glutamate exchanger;glutathione;lens epithelial cells;SLC7A11;system xc–