Immunoelectron Microscopic Observation of Chicken Glucagon-Like Peptide(GLP)-1-Containing Cells in Tissues Derived from Thin Section, Paraffin Block andConventional Method

[摘要] References(27)Cited-By(3)The purpose of the present study was to investigate the possibility ofimmunoelectron microscopic observation of endocrine cells in paraffin-embedded tissues.The procedure, which involves reprocessing from sliced tissues and immunohistochemicalstaining by colloidal-gold immunolabeling of paraffin sections from paraffin blocks, wasable to reveal the fine characteristics of secretory granules containing glucagon-likepeptide-1. Morphometric analyses of the secretory granules showed no significantdifferences between the reprocessing procedure and a conventional post-embeddingprocedure, which was performed as a control. The reprocessing procedure has someadvantages besides providing information on secretory granules containing the amino acidpeptide. For example, the same cell can be observed under both a light microscope and theelectron microscope. In addition, the high-electron densities of silver-enhanced goldparticles are easily recognized, and the boundary between the profile of the granules andthe immunogold labeling is clearly shown at the electron microscopic level. Furthermore,the procedure, which is inexpensive and does not require special devices, can effectivelyuse precious samples that are already paraffin-embedded and unable to be obtained twice,such as the case for endangered animals and rare pathological tissues. To the best of ourknowledge, the present study is the first to report the advantages of the reprocessingmethod for sliced paraffin sections of gut endocrine cells.

[学科分类] 兽医学

[关键词] glucagon-like peptide-1;immunocytochemistry;paraffin-embedded tissue;reprocessing method;transmission electron microscope