IL-10 Gene Polymorphisms and Their Association with Immune Traits in FourRabbit Populations

[摘要] References(27)Interleukin-10 (IL-10) hasbeen recently identified as a multifunctional cytokine, because of its close link withimmunoregulation and anti-inflammatory responses. This study investigated the associationof IL-10 genetic polymorphisms with the immune traits of New Zealand white rabbits (N-W),Fujian yellow rabbits (F-Y) and their reciprocal crosses (N-Y and Y-N, respectively). SNPson five exons of the IL-10 gene were genotyped in 204 healthy rabbits via PCR-SSCP and DNAsequencing. Two SNPs (A1435G and G1519A, both were synonymous mutations) and six genotypes(AA, BB, CC, AB, AC and BC) were found on exon 3 and one SNP (T base insertion betweenloci 2532 and 2533, which caused a frameshift mutation), and three genotypes (OO, TT andTO) were present on exon 4. Allele A was the most frequent allele on exon 3 (from 0.548 to0.771), whereas O was the most frequent on exon 4 (from 0.808 to 0.968). These fourpopulations were all in Hardy-Weinberg equilibrium on both exon3 and exon4. Associationanalysis between polymorphisms and immune parameters showed that SNPs on exon 3 weresignificantly associated with immune traits, while SNP on exon 4 may not significantlyaffect immune traits, but the mechanism is yet to be further studied.

[学科分类] 兽医学

[关键词] exon;IL-10 gene;immune parameters;PCR-SSCP;polymorphism