Generation of Monoclonal Autoantibodies from Babesiarodhaini-Infected Mice

[摘要] References(20)The presence ofanti-erythrocyte autoantibodies in animals infected with various Babesiaspecies is well reported. However, the pathogenesis of autoantibodies in babesiosis ispoorly understood. Here, we demonstrated that anti-erythrocyte immunoglobulin (Ig) M andIgG were present in B. rodhaini-infected mice at 6 and 8 days afterinfection, respectively. Furthermore, we generated monoclonal antibodies againsterythrocyte antigen from B. rodhaini-infected mice. Five clones weregenerated. By Western blotting analysis using whole erythrocyte antigens, one clonereacted with a broad band around 90�?150 kDa, and the 2 clones reacted with a band largerthan 150 kDa. B. rodhaini-infected mice and/or autoreactive monoclonalantibodies established in this study might be a powerful tool for in vivopathogenesis studies of autoantibody development in infectious diseases.

[学科分类] 兽医学

[关键词] autoantibody;Babesiosis;erythrocyte antigen;immune-mediated anemia;monoclonal antibody