Induction of Beige-Like Adipocytes in 3T3-L1 Cells

[摘要] References(47)Cited-By(7)There are two types of brown adipocytes: classical brown adipocytes that formthe brown fat depots and beige adipocytes that emerge in the white fat depots. Beigeadipocytes have a low level of uncoupling protein 1 (Ucp1) expression in the basal state,but Ucp1 expression is increased in response to β adrenergic receptor activation. Thepresent study explored the factors responsible for the differentiation of 3T3-L1 whitepreadipocytes to beige adipocytes. Significant expression of Ucp1 was notdetected under any tested conditions in the absence of isoproterenol (Iso), an agonist ofβ adrenergic receptor. Iso-induced Ucp1 expression was significantlyhigher in the cells treated with a mixture of triiodothyronine (T3) and3-isobutyl-1-methylxanthine (IBMX) for days 0�?8 than in the control cells. Chronic IBMXtreatment was indispensable for the enhanced Iso-induced Ucp1 expression,and treatment with additional rosiglitazone (Rosi) for days 0�?8 further increased theUcp1 expression. Recently, genes were identified that are predominantlyexpressed in beige adipocytes, which were induced from stromal vascular cells in white fatdepots. However, the expression levels of the beige adipocyte-selective genes in theadipocytes induced by the mixture of T3, IBMX and Rosi did not differ fromthose in the control adipocytes. The present study indicates that 3T3-L1 cells candifferentiate to beige-like adipocytes by prolonged treatment with the mixture ofT3, IBMX and Rosi and that the gene expression profile of the adipocytes isdistinct from those previously induced from white fat depots.

[学科分类] 兽医学

[关键词] adipocyte;beige adipocyte;cell culture;cellular differentiation;Ucp1