Prevalence and Molecular Analyses of HemotrophicMycoplasma spp. (Hemoplasmas) Detected in Sika Deer(Cervus nippon yesoensis) in Japan

[摘要] References(17)Cited-By(1)Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocyticbacteria that cause infectious anemia in several mammalian species. The prevalence ofhemoplasma species was examined by screening and species-specific PCR using blood samplescollected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Usingspecies-specific PCR, 12 and 17 samples were positive for ‘CandidatusMycoplasma haemocervae�? and ‘Candidatus M. erythrocervae�?, respectively.Sequencing and phylogenetic trees of those three genes indicate that the‘Candidatus M. haemocervae�? and ‘Candidatus M.erythrocervae�? detected in Japanese deer are potentially different species from thecervine hemoplasma found in deer from America and Brazil.

[学科分类] 兽医学

[关键词] Cervus nippon yesoensis;hemoplasma;Japan;phylogeny