Prevalence and Genotyping of Cryptosporidium spp. in FarmAnimals in Egypt

[摘要] References(54)Cited-By(8)In this study, we examined theprevalence and molecular characteristics of Cryptosporidium in buffalo,dairy cattle and sheep in different farms at Kafr El Sheikh Province, Egypt. Rectal fecalsamples, including 466 samples from buffalo, 1697 from cattle and 120 from sheep, werecollected from different ages and screened by modified Ziehl-Neelsen acid-fast microscopyfor detection of Cryptosporidium oocysts. All studied farms werepositives with an overall prevalence of 1.29% in buffalo (4.17% in claves versus 0.48% inadults), 7.07% in cattle (6.90% in calves versus 10.20% and 6.10% in heifers and adults,respectively) and 2.50% in sheep (4.40% in lambs versus 1.30% in adults). PCR-RFLPanalyses of small-subunit rRNA genes from positive specimens revealed the occurrence ofC. parvum and C. ryanae in buffalo; C.parvum, C. ryanae, C. bovis and C.andersoni in cattle and only C. xiaoi in sheep. Genotypesdistribution showed that C. ryanae was the dominant species (60.0%)followed by C. parvum (40.0%) in buffalo calves. Meanwhile, in cattlecalves, C. parvum was the commonest species (74.23%) followed byC. ryanae (16.10%) and C. bovis (9.70%). Subtyping ofC. parvum based on sequence analysis of the polymorphic 60 kDaglycoprotein gene locus showed the presence of subtypes IIdA20G1 and IIaA15G1R1 in bothbuffalo and cattle calves, addressing the potential role of calves in zoonoticcryptosporidiosis in Egypt.

[学科分类] 兽医学

[关键词] Cryptosporidium;Egypt;genotyping;subtyping;zoonosis