Expression of Adrenergic and Cholinergic Receptors in Murine RenalIntercalated Cells

[摘要] References(50)Cited-By(2)Neurons influence renal function and help to regulate fluid homeostasis, bloodpressure and ion excretion. Intercalated cells (ICCs) are distributed throughout the renalcollecting ducts and help regulate acid/base equilibration. Because ICCs are located amongprincipal cells, it has been difficult to determine the effects that efferent nerve fibershave on this cell population. In this study, we examined the expression ofneurotransmitter receptors on the murine renal epithelial M-1 cell line. We found that M-1cells express a2 and b2 adrenergic receptor mRNA and the b2 receptor protein. Further, b2receptor-positive cells in the murine cortical collecting ducts also express AQP6,indicating that these cells are ICCs. M-1 cells were found to express m1, m4 and m5muscarinic receptor mRNAs and the m1 receptor protein. Cells in the collecting ducts alsoexpress the m1 receptor protein, and some m1-positive cells express AQP6.Acetylcholinesterase was detected in cortical collecting duct cells. Interestingly,acetylcholinesterase-positive cells neighbored AQP6-positive cells, suggesting thatprincipal cells may regulate the availability of acetylcholine. In conclusion, our datasuggest that ICCs in murine renal collecting ducts may be regulated by the adrenergic andcholinergic systems.

[学科分类] 兽医学

[关键词] autonomic nervous system;nephrology;parasympathetic;renal epithelium;sympathetic