Anti-Inflammatory Effects of Mannanase-Hydrolyzed Copra Meal in a PorcineModel of Colitis

[摘要] References(27)Cited-By(3)We evaluated the anti-inflammatory activity of mannanase-hydrolyzed copra meal (MNB),including β-1,4-mannobiose (67.8%), in a dextran sodium sulfate (DSS)-induced porcinemodel of intestinal inflammation. In the DSS-positive control (POS) and MNB treatment(MCM) groups, DSS was first administered to piglets via intragastric catheter for 5 days,followed by 5 days administration of saline or MCM. A negative control group (NEG)received a saline alternative to DSS and MNB. Inflammation was assessed by clinical signs,morphological and histological measurements, gut permeability and neutrophil infiltration.Local production of TNF-α and IL-6 were analyzed by ELISA, colonic and ileal inflammatorygene expressions were assessed by real time RT-PCR, and CD4+CD25+ cell populations wereanalyzed by flow cytometry. Crypt elongation and muscle thickness, D-mannitol gutpermeation, colonic expression of the inflammatory mediators TNF-α and IL-6 andmyeloperoxidase activity were significantly lower in the MCM group than in that of POSgroup. The mRNA levels of ileal IL-1β, IL-6, IL-17 and TNF-α were significantly lowerfollowing MCM treatment than with POS treatment.MNB exerts anti-inflammatory activityin vivo, suggesting that MNB is a novel therapeutic that may providerelief to human and animals suffering from intestinal inflammation.

[学科分类] 兽医学

[关键词] 4-mannobiose;β-1;anti-inflammation;colitis;porcine