Molecular Cloning of cDNA for Equine Ovarian Inhibin/Activin βA Subunit

[摘要] References(19)Cited-By(6)cDNAs encoding equine inhibin/activin βA subunit were isolated from an equine follicle cDNA library and characterized. Using primers based on the rat inhibin/activin βA subunit cDNA sequence, a RT-PCR was performed to generate the probe for screening. Four positive clones were isolated. Analysis of the nucleotide sequence of these clones revealed that two pairs of identical clones were present, Eq-βA-1 (0.9 kb) and Eq-βA-2 (1.5 kb). Eq-βA-2 clone contained a complete open reading frame encoding 426amino acids. The deduced amino acid sequence of equine inhibin/activin βA subunit showed high similarity (>90%) to those of five other mammalian species. Northern blot analysis revealed that placenta from mare on day 180 of pregnancy contained a 1.5 kb inhibin/activin βA subunit mRNA.

[学科分类] 兽医学

[关键词] activin;cDNA cloning;equine;inhibin;ovary