Effect of Extracellular Matrix on Two Cell Lines Established from Mongolian Gerbil's (Meriones unguiculatus) Malignant Melanoma

[摘要] References(27)Cited-By(1)We investigated the effect of extracellular matrix (ECM) on the adhesion, morphology and proliferation of two Mongolian gerbil's melanoma cell lines (MGM-S, MGM-A) which differed in malignancy. Fibronectin matrix was detected on the cell surface of MGM-S by immunofluorescence techniques. MGM-A cells, showing a multipolar shape, were widely spread over the dishes coated with fibronectin, laminin or type IV collagen. The adhesion rate of MGM-A cells to ECM components was higher compared to that in controls. Attachment to laminin was conspicuously promoted. The effect of laminin on MGM-A cell growth was also the highest among the substrates examined. On the other hand, these ECM components did not affect MGM-S cells. These results suggest that laminin and fibronectin may participate in the malignancy of MGM cell lines.

[学科分类] 兽医学

[关键词] tibronectin;laminin;malignancy;melanoma cell line;Mongolian gerbil