Immunohistochemical Microquantitation Method for Type I Collagen in Kidney Histological Section of the Rats

[摘要] References(9)Cited-By(6)An immunohistochemical micromethod for quantitation of type I collagen in frozen histological section of rat kidney was developed. A principle of this method is enzyme-linked immunosorbent assay (ELISA). The intensity of immunoreactivity of the section to an anti-type I collagen antibody was quantitated, and then the total protein content in the same section was measured colorimetrically. Type I collagen content was expressed as mg of type I collagen per g of total protein. In this micromethod, the minimum area and optimum thickness of the section were 5 mm2 and 10μm, respectively. Type I collagen content in the rat kidney section was 3.02±0.12 mg/g. This method should be useful for diagnosis of renal failure.

[学科分类] 兽医学

[关键词] immunohistochemical quantitation;kidney;type I collagen