Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolatedfrom migratory wild geese in Japan

[摘要] References(24)Cited-By(5)An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) wasbiologically, serologically and genetically characterized. APMV/Shimane67 showed typicalparamyxovirus morphology on electron microscopy. On hemagglutination inhibition test,antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes andvice versa. The fusion (F) protein gene of APMV/Shimane67 contained1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids.The cleavage site of F protein contained a pair of single basic aminoacid (VRENR/L). The nucleotide and deduced amino acid sequences of the F geneof APMV/Shimane67 had relatively low identities (42.9�?62.7% and 28.9�?67.3%, respectively)with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related toNDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggestthat APMV/Shimane67 is a new APMV serotype, APMV-13.

[学科分类] 兽医学

[关键词] APMV;avian paramyxovirus;F gene;phylogenetic analysis;serotype