Prevalence of porcine reproductive and respiratory syndrome virus detectionin aborted fetuses, mummified fetuses and stillborn piglets using quantitative polymerasechain reaction

[摘要] References(29)The objective of the present study was to investigate the prevalence of porcinereproductive and respiratory syndrome (PRRS) virus detection in aborted fetuses (n=32),mummified fetuses (n=30) and stillborn piglets (n=27) from 10 swine herds in Thailandusing quantitative polymerase chain reaction (qPCR). Pooled organs and umbilical cord fromeach fetus/piglet were homogenized and subjected to RNA extraction and cDNA synthesis. TheqPCR was carried out on the ORF7 of the PRRS viral genome using fluorogenic probes foramplified product detection. The results revealed that 67.4% (60/89) of the specimenscontained PRRS virus. The virus was found in 65.6% (21/32) of aborted fetuses, 63.3%(19/30) of mummified fetuses and 74.1% (20/27) of stillborn piglets(P=0.664). Genotype 1, genotype 2 and mixed genotypes of PRRS virus weredetected in 19.1% (17/89), 25.8% (23/89) and 22.5% (20/89) of the specimens, respectively(P=0.316). PRRS virus antigen was retrieved from bothnon-PRRS-vaccinated herds (68.2%, 45/66) and PRRS-vaccinated herds (65.2%, 15/23)(P=0.794). These findings indicated that these specimens are importantsources of the PRRS viral load and the viral shedding within the herd. Thus, intensivecare on the routine management of dead fetuses and stillborn piglets in PRRSvirus-positive herds should be emphasized.

[学科分类] 兽医学

[关键词] mummy;PRRS;qPCR;reproduction;swine