Isolation and characterization of arylacetamide deacetylase in cynomolgusmacaques

[摘要] References(23)Arylacetamide deacetylase (AADAC), a microsomal serine esterase, hydrolyzes drugs, suchas flutamide, phenacetin and rifampicin. Because AADAC has not been fully investigated atmolecular levels in cynomolgus macaques, the non-human primate species widely used in drugmetabolism studies, cynomolgus AADAC cDNA was isolated and characterized. The deducedamino acid sequence, highly homologous (92%) to human AADAC, was more closely clusteredwith human AADAC than the dog, rat or mouse ortholog in a phylogenetic tree.AADAC was flanked by AADACL2 andSUCNR1 in the cynomolgus and human genomes. Moreover, relativelyabundant expression of AADAC mRNA was found in liver and jejunum, the drug-metabolizingorgans, in cynomolgus macaques, similar to humans. The results suggest molecularsimilarities of AADAC between cynomolgus macaques and humans.

[学科分类] 兽医学

[关键词] arylacetamide deacetylase;cynomolgus monkey;drug metabolism;genome;tissue expression