Genetic and antigenic analysis of Chlamydia pecorum strainsisolated from calves with diarrhea

[摘要] References(27)Chlamydia pecorum (designated 22�?58) was isolated in 2010 inHmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis inYamaguchi Prefecture, Japan. Immunohistochemical staining identified C.pecorum positive reactions in the jejunal villi. C. pecorum,designated 24�?100, was isolated from the feces of a calf with diarrhea in another farm inYamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titersagainst C. pecorum was confirmed in paired sera. Nucleotide sequenceidentities of omp1 genes of the 2 isolates were 100%. The isolates weregenetically and antigenically more closely related to C. pecorumBo/Yokohama strain isolated from cattle with enteritis in Japan than to the otherprototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated fromsheep with polyarthritis. These results indicate that C. pecorum strainssimilar to 22�?58 and 24�?100 might be endemic in Yamaguchi Prefecture and cause entericdisease in cattle.

[学科分类] 兽医学

[关键词] cattle;Chlamydia pecorum;diarrhea