Analysis of L-type amino acid transporter in canine hepatocellularcarcinoma

[摘要] References(38)Analysis of L-type amino acid transport expression of hepatocellular carcinoma cells(HCCs) of the dog was performed. The leucine transport activity of canine HCCs was 0.628 ±0.018 nmol/mg protein/min. The inhibitor of LAT 2-aminobicyclo[2.2.1]heptane-2-carboxylicacid (BCH) reduced 90% of the activity at 1 mM. The deduced amino acid sequences of canineLAT2, LAT3 and LAT4 were well conserved in mammalians, exhibiting 89, 88 and 77% homology,respectively. RT-PCR revealed distinct LAT1 expression compared with normal hepatocytes.Western blotting analysis confirmed the potent LAT1 expression in canine HCCs but nothepatocytes, and real-time RT-PCR analysis indicated that canine HCCs possessed 28 timeshigher LAT1 expression than hepatocytes. These results indicated that the leucinetransport activity of canine HCCs was due to LAT1.

[学科分类] 兽医学

[关键词] canine;hepatoma;LAT;transporter