A Ca2+-Dependent Phosphorylclcholine-Binding Protein in Chicken Serum

[摘要] References(24)A Ca2+-dependent phosphorylcholine (PC)-binding protein in normal chicken serum was purified by affinity chromatography on p-aminophenyl PC-Sepharose 4B followed by gel filtration on Sephacryl S-300. In gel filtration, the isolated PC-binding protein was eluted in a symmetrical protein peak at the position of approximately 100, 000. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the protein was resolved into two protein bands of 31, 000 and 38, 000 under nonreducing conditions and of 40, 000 and 46, 000 under reducing conditions. These results suggest that chicken serum PC-binding protein may be composed of two different subunits which contain in intrachain disulfide bonds.

[学科分类] 兽医学

[关键词] chicken serum protein;isolation;phosphorylcholine-binding