Application of a Recombinant Baculovirus Expressing Hantavirus Nucleocapsid Protein as a Diagnostic Antigen in IFA Test : Cross Reactivities among 3 Serotypes of Hantavirus which Causes Hemorrhagic Fever with Renal Syndrome (HFRS)

[摘要] References(14)Cited-By(18)Recombinant baculovirus-infected insect cells which expressed recombinant protein, analogous to the nucleocapsid protein (NP) of Seoul type hantavirus, strain SR-11 (rNP-SR-Sf9) were applied to the indirect immunofluorescent antibody (IFA) test. The rNP-SR-Sf9 reacted with anti NP MAb clones which recognized strain specific or hantavirus common epitopes in the IFA test. The recombinant antigen was detected by antibodies to 3 serotypes of hantaviruses (Hantaan 76-118, SR-11, Puumala). Antibody titers of a group of experimentally infected mouse and urban rat sera using rNP-SR-Sf9 and strain SR-11-infected Vero cell antigens were mutual correlative. These results indicated that the rNP-SR-Sf9 were an effective and safety substitute for Vero E6 cells in the IFA test for serosurveys of hantavirus infection.

[学科分类] 兽医学

[关键词] baculovirus;diagnosis;hantavirus