Molecular Cloning and Immunological Analysis of Immunodominant Piroplasm Surface Proteins of Theileria sergenti and T. buffeli

[摘要] References(15)Cited-By(16)cDNA libraries of Theileria sergenti and T. buffeli piroplasms were constructed in λ gt11 and screened with rabbit anti-piroplasm sera. A major antigen of T. sergenti (33 kDa) and that of T. buffeli (34 kDa) was identified from the recombinant phages by using recombinant antigen-selected monospecific antibodies. The reactivities of the cloned proteins with rabbit antisera, infected calf sera and mouse monoclonal antibody suggested that the 33 and 34 kDa proteins expressed species-common and species-specific epitopes. The DNA probes from these recombinant clones showed species-specific hybridizatioins in Southern blotting with genomic DNA from piroplasms. These results indicate that the Japanese T. sergenti can be distinguishable from the Australian T. buffeli with regard to a polymorphism of the major immunodominant proteins of piroplasm.

[学科分类] 兽医学

[关键词] cDNA cloning;Theileria buffeli;Theileria orientalis;Theileria sergenti;λ gt11