The use of autologous neurogenically-induced bone marrow-derived mesenchymalstem cells for the treatment of paraplegic dogs without nociception due to spinaltrauma

[摘要] References(47)The aim of this study was to investigate the effects of percutaneous transplantedautologous neurogenically-induced bone marrow-derived mesenchymal stem cells (NIBM-MSCs)in paraplegic dogs without deep pain perception (DPP) secondary to external spinal trauma.Thirteen client owned dogs that had failed in improvement neurologically at least 42 daysafter conservative management, decompression and decompression-stabilization were includedin the study. Each dog received two doses of autologous 5.0 × 106 NIBM-MSCssuspension, which were positive to 2�?,3�?-Cyclic-nucleotide-3�?-phosphodiesterase (CNPase)and Microtubule-associated protein 2 (MAP-2), as well as to Glial fibrillary acidicprotein (GFAP) and beta III tubulin. The cells were injected into the spinal cord throughthe hemilaminectomy or laminectomy defects percutaneously with 21 days interval for 2times. The results were evaluated using Texas Spinal Cord Injury Scale (TSCIS),somatosensory evoked potentials (SEP) and motor evoked potentials (MEP) at the admissiontime, cell transplantation procedures and during 2, 5, 7 and 12th months after the secondcell transplantation. Improvement after cell transplantation in gait, nociception,proprioception, SEP and MEP results was observed in just 2 cases, and only gait scoreimprovement was seen in 6 cases, and no improvement was recorded in 5 cases. Allprogresses were observed until 2nd month after the second cell transplantation, however,there was no improvement after this period. In conclusion, percutaneous transplantation ofautologous NIBM-MSCs is a promising candidate modality for cases with spinal cord injuryafter spinal trauma and poor prognosis.

[学科分类] 兽医学

[关键词] autologous stem cells;external trauma;paraplegia;spinal cord injury;spinal stabilization