Analyses of a satiety factor NUCB2/nesfatin-1; gene expressions andmodulation by different dietary components in dogs

[摘要] References(36)Cited-By(1)Supplementary materials(2)Nesfatin-1 is an anorexic peptide derived from a precursor, nucleobindin-2 (NUCB2), whichis distributed in various organs, coexists with ghrelin in the gastric X/A-like cells andclosely relates to an appetite control in rodents and humans. Nesfatin-1 may be asignificant factor addressing the satiety also in veterinary medicine, however, there arefew reports about nesfatin-1 in dogs. In the present study, we detected canineNUCB2/nesfatin-1 mRNA in various tissues, especially abundant in pancreas,gastrointestinal tracts, testis and cerebellum. We examined circulating nesfatin-1concentrations and NUCB2/nesfatin-1 mRNA expressions in upper gastrointestinal tracts(gastric corpus, pyloric antrum and duodenum) in dogs fed on different types of diets.Plasma nesfatin-1 concentrations in the dogs were approximately 4ng/ml and they did not change after feeding throughthe study, however, NUCB2/nesfatin-1 mRNA expressions in pyloric antrum were 1.84-foldhigher in the dogs fed on a High fiber/High protein diet (P<0.001),1.48-fold higher in the dogs fed on a High fat/Low protein diet(P<0.05) and 1.02-fold higher in the dogs fed on a Low fat/Highcarbohydrate diet (not significant) comparing to those on a control diet. It was concludedthat High fiber/High protein and High fat/Low protein diets increased NUCB2/nesfatin-1production in canine gastrointestinal tracts. These results may set the stage for furtherinvestigations of canine NUCB2/nesfatin-1, which may relate to satiety effects indogs.

[学科分类] 兽医学

[关键词] anorexic peptide;canine;nesfatin-1;nucleobindin-2;obesity