Simultaneous subtyping and pathotyping of avian influenza viruses in chickensin Taiwan using reverse transcription loop-mediated isothermal amplification andmicroarray

[摘要] References(24)The H6N1 avian influenza virus has circulated in Taiwan for more than 40 years. Thesporadic activity of low pathogenic H5N2 virus has been noted since 2003, and highlypathogenic H5N2 avian influenza virus has been detected since 2008. Ressortant virusesbetween H6N1 and H5N2 viruses have become established and enzootic in chickens throughoutTaiwan. Outbreaks caused by Novel highly pathogenic H5 avian influenza viruses whose HAgenes were closely related to that of the H5N8 virus isolated from ducks in Korea in 2014were isolated from outbreaks in Taiwan since early 2015. The avian influenza virusinfection status is becoming much more complicated in chickens in Taiwan. Thisnecessitates a rapid and simple approach to detect and differentiate the viruses thatprevail. H6N1, H5N2 and novel H5 viruses were simultaneously subtyped and pathotyped inthis study using reverse transcription loop-mediated isothermal amplification andmicroarray, with detection limits of 10°, 101 and 10° viral copy numbers,respectively. The microarray signals were read by the naked eye with no expensiveequipment needed. The method developed in this study could greatly improve avian influenzavirus surveillance efficiency.

[学科分类] 兽医学

[关键词] avian influenza virus;microarray;pathotyping;reverse transcription loop-mediated isothermal amplification;subtyping