Relationship of bovine TNF-α gene polymorphisms with therisk of bovine tuberculosis in Holstein cattle

[摘要] References(40)Many studies suggest significant genetic variation in the resistance of cattle and humansto infection with Mycobacterium bovis (M. bovis), thecausative agent of zoonotic tuberculosis. TNF-α promotes inflammation and inducesapoptosis in response to mycobacterial infection. The aim of the present study was toinvestigate the influence of single nucleotide polymorphisms of the TNF-αgene on bovine tuberculosis (bTB) susceptibility. We genotyped the TNF-αgene in 74 bTB-infected Holstein cows and 90 healthy control animals. The influence in theexon 3 region of TNF-α polymorphisms on bTB susceptibility wassubsequently investigated by association analysis. Our finding demonstrated that theg.27534932A>C polymorphism of the TNF-α is associated with bTB inHolstein cattle. The susceptibility of cattle with the g.27534932A>C genotype comparedwith the CC genotype was 4.11-fold (95% CI, 1.27�?13.36; P=0.02) higher.The g.27534932A>C polymorphism located in exon 3 of the TNF-α gene,and the functional consequence was missense. The deduced amino acid sequence for theprotein product revealed an arginine to serine conversion at position 159, which mayaffect initiation of protein synthesis and disrupt normal TNF-α functionthat protects animals against mycobacterial infection. A significant association wasobserved with the A allele as a risk factor for bTB susceptibility (OR, 3.84; 95% CI,1.21�?12.17; P=0.02). In conclusion, this is the first report showing thatthe g.27534932A>C polymorphism may contribute to TNF-α-mediated bTBsusceptibility.

[学科分类] 兽医学

[关键词] bovine tuberculosis;polymorphism;susceptibility;TNF-α