Development of a mouse-feline chimeric antibody against feline tumor necrosisfactor-alpha

[摘要] References(25)Supplementary materials(1)Feline infectious peritonitis (FIP) is a fatal inflammatory disease caused by FIP virusinfection. Feline tumor necrosis factor (fTNF)-alpha is closely involved in theaggravation of FIP pathology. We previously described the preparation of neutralizingmouse anti-fTNF-alpha monoclonal antibody (mAb 2�?4) and clarified its role in the clinicalcondition of cats with FIP using in vitro systems. However,administration of mouse mAb 2�?4 to cat may lead to a production of feline anti-mouseantibodies. In the present study, we prepared a mouse-feline chimeric mAb (chimeric mAb2�?4) by fusing the variable region of mouse mAb 2�?4 to the constant region of felineantibody. The chimeric mAb 2�?4 was confirmed to have fTNF-alpha neutralization activity.Purified mouse mAb 2�?4 and chimeric mAb 2�?4 were repeatedly administered to cats, and thechanges in the ability to induce feline anti-mouse antibody response were investigated. Inthe serum of cats treated with mouse mAb 2�?4, feline anti-mouse antibody production wasinduced, and the fTNF-alpha neutralization effect of mouse mAb 2�?4 was reduced. Incontrast, in cats treated with chimeric mAb 2�?4, the feline anti-mouse antibody responsewas decreased compared to that of mouse mAb 2�?4-treated cats.

[学科分类] 兽医学

[关键词] cat;chimeric antibody;monoclonal antibody;tumor necrosis factor-alpha