Anesthetic effects of a combination of medetomidine, midazolam andbutorphanol on the production of offspring in Japanese field vole, Microtusmontebelli

[摘要] References(33)Pentobarbital sodium (Somnopentyl) can induce surgical anesthesia with a strong hypnoticeffect that causes loss of consciousness. Animals have been known to die duringexperimental surgery under anesthesia with Somnopentyl, causing it to be declaredinadequate as a general anesthetic for single treatment. An anesthetic combination of 0.3mg/kg medetomidine, 4.0 mg/kg midazolam and 5.0 mg/kg butorphanol (M/M/B:0.3/4/5) wasreported to induce anesthesia for a duration of around 40 min in ICR mice; similaranesthetic effects were reported in both male and female BALB/c and C57BL/6J strains ofmice. However, the anesthetic effects of this combination in Japanese field vole,Microtus montebelli, remain to be evaluated. In the present study, weassessed the effects of Somnopentyl and different concentrations of anesthetic combination(M/M/B:0.3/4/5, 0.23/3/3.75 or 0.15/2/2.5) in Japanese field voles, by means of anestheticscores. We also examined effect of these anesthetics on production of offspring. Death ofthe animals was observed only with Somnopentyl. The anesthetic score of Somnopentyl waslower than those of the other anesthetics, although there were no significant differencesin duration, body weight and frequency of respiratory among the evaluated anesthetics.Abortion rate with Somnopentyl was significantly higher than that with theM/M/B:0.23/3/3.75 combination, although there was no significant difference in the numberof offspring between two. In conclusion, results of this study provide basic informationfor achieving appropriate anesthetic concentrations in addition to indicating a new, safeand effective surgical anesthetic for Japanese field voles.

[学科分类] 兽医学

[关键词] anesthetic combination;atipamezole;Japanese field vole;offspring;Somnopentyl