Analysis of lipoprotein profiles of healthy cats by gel-permeationhigh-performance liquid chromatography

[摘要] References(23)Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionallyused to obtain lipoprotein profiles of animals. We recently applied high-performanceliquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymaticsystem to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as amethod to obtain a feline lipoprotein profile. The lipoprotein profiles showed large andsmall peaks, which corresponded to high-density lipoprotein (HDL) and low-densitylipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) andchylomicron (CM) were only marginally detected. This profile was very similar to that ofdogs reported previously. Healthy cats also had a small amount of cholesterol-richparticles distinct from the normal LDL or HDL profile. There was no difference inlipoprotein profiles between the sexes, but males had a significantly larger LDL particlesize (P=0.015). This study shows the feasibility of GP-HPLC for obtainingaccurate lipoprotein profiles with small sample volumes and provides valuable referencedata for healthy cats that should facilitate diagnoses.

[学科分类] 兽医学

[关键词] cat;HPLC;lipoprotein profile;triglyceride