The epidemiological survey for atovaquone resistant related gene ofBabesia gibsoni in Japan

[摘要] References(13)In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variantpopulation was measured by using allele-specific real-time PCR. Although the mechanism ofatovaquone against B. gibsoni has not been clearly identified, it isreported that the mitochondria cytochrome b gene of theatovaquone-resistant B. gibsoni had a single-nucleotide substitution atnt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I).In this study, 3/73 samples showed over 5% M121I variant population. Although the M121Ivariant population is a low percentage, it runs the risk of spreading drug-resistantparasites. It is important to prevent the spread of drug-resistance, so we need to gatherinformation about this at regular intervals.

[学科分类] 兽医学

[关键词] allele-specific real-time PCR;Babesia gibsoni;drug resistance;M121I variant population