Nuclear magnetic resonance-based serum metabolic profiling of dairy cows withfootrot

[摘要] References(38)Footrot is a debilitating and contagious disease in dairy cows, caused by theGram-negative anaerobe Dichelobacter nodosus. 1H-NMR (nuclearmagnetic resonance)-based metabolomics has been previously used to understand thepathology and etiology of several diseases. The objective of this study was tocharacterize serum from dairy cows with footrot (n=10) using 1H-NMR-basedmetabolomics and chemometric analyses. 1H-NMR spectroscopy with multivariatepattern recognition (principal component analysis and orthogonal partial least-squaresdiscriminant analysis) was performed to identify biomarkers in cows with footrot (F) andhealthy controls (C). 1H-NMR analysis facilitated the identification of 21metabolites. Among these metabolites, 4 metabolites were higher and 17 metabolites werelower in the F group than in the C group. The serum levels of 5 metabolites weresignificantly different (P<0.05) between the two groups. The resultsrevealed that cows with footrot have altered carbohydrate, amino acid, lipid and energymetabolic pathways. Metabolomic approaches are a clinically useful diagnostic tool forunderstanding the biochemical alterations and mechanisms of several diseases.

[学科分类] 兽医学

[关键词] 1H-NMR;dairy cow;footrot;metabolomics;pathway