Bisphenol A glucuronide/sulfate diconjugate in perfused liver ofrats

[摘要] References(23)Cited-By(2)In isolated hepatocytes, the environmental estrogen bisphenol A (BPA) is metabolized intoa mono-glucuronide and a glucuronide/sulfate diconjugate. Little is known about the fateof the diconjugate in the liver. The present study focused on the metabolism anddispostion of BPA diconjugate in the liver using a perfusion method. In Sprague-Dawleyrats, BPA (15,150 or 1,500 nmol) was applied into the liver. In male rats, the infused BPAwas conjugated to both glucuronide and a diconjugate during passage through the liver. Thediconjugate was observed at high-dose application of the substrate. In female rats, thechemical was conjugated almost exclusively to the glucuronide in all doses utilized inthis study. In both the male and female rats, the resultant metabolites werepreferentially excreted into the bile. These results suggest that BPA is conjugatedprimarily to mono-glucuronide in rat liver; and that in males, diconjugate productionoccurs under conditions of high-dose exposure to BPA.

[学科分类] 兽医学

[关键词] bisphenol A;diconjugation;glucuronide conjugation;liver perfusion;sulfate conjugation