Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes

[摘要] ENGLISH ABSTRACT:This study describes:I. The cloning of the CVP 19 gene and construction of the intracellular expressionvector pPIC3.5K-CYP19.II. The transformation of the yeast, Pichia pastoris with the constructed vector.III. The expression ofP450arom in Pichia pastoris.IV. The determination of enzyme activity and isolation of the protein from the Pichiapastoris cells.V. The expression of P450c 17 in Pichia pastoris.VI. The determination of kinetic constants for the conversion of progesterone to170H-progesterone and 160H-progesterone by P450c17.

[发布机构] Stellenbosch University