Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up

[摘要] ENGLISH ABSTRACT: This study describes:a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsinb) Re-engineering / development of a new method for purifying trypsin and α-chymotrypsin that delivered higher product yields and improved control exercised over the process by investigating:i. Extraction methodsii. Centrifugationiii. Ultrafiltrationiv. Chymotrypsinogen and trypsin crystallizationv. Column chromatographyvi. Investigation into different raw material sources for pancreatic enzyme productionc) Development of kinetic and ELISA testing methodologies for in-process QC analysis.

[发布机构] Stellenbosch University