We present a new record of the culpeo (Lycalopex culpaeus) from southwestern Colombia, and discuss the previous records and localities of the species in the country. The species is known from three confirmed localities; two of them are represented by voucher specimens and the third is presented here based on a photography obtained on 2011. All confirmed localities are located in the Nudo de Los Pastos, Department of Nariño, southwestern Colombia. Other localities and records of the species in literature are in need of more evidence that corroborate the presence of the species in these areas, while the records from the Central Cordillera in the Departments of Valle del Cauca, Tolima, and from the Western Cordillera in the Department of Cauca are considered erroneous.
