已收录 272612 条政策
 政策提纲
  • 暂无提纲
Analysis of the effects of the plant growth promoting substances GR24 and smoke water on abiotically stressed Nicotiana benthamiana seedlings
[摘要] ENGLISH ABSTRACT: Almost all processes during the life of a plant are affected by the environment.Changes in phytohormone, metabolite and protein levels follow in response tochanges in the environment. Plant growth promoting substances can stimulatechanges at these levels to facilitate increased plant growth and yields above whatthe plant would normally establish. In this study, the effects of two growth promotingsubstances, smoke water (SW) derived from bubbling smoke from the burning ofplant material through water, and a synthetic strigolactone analogue, GR24, on plantgrowth and architecture, as well as the proteome and metabalome of salt stressedNicotiana benthamiana seedlings were investigated. Physiological studies wereconducted to identify the effects of the growth substances on salt stressed seedlingsin a tissue culture system. Under non-stress conditions, SW treatment increasedseedling fresh mass, root length and leaf area. Under salt stress conditions (100mM and 150 mM NaCl), SW increased fresh mass, root length, leaf number andlateral root number significantly. Under non-stress conditions, GR24-treatedseedlings showed increased fresh mass, leaf number and area and root length.When GR24-treated seedlings were placed under salt stress, the seedlings showedsignificant increases in fresh mass, leaf number and lateral root number, but onlymarginal increases in root length and leaf area. Despite these similarities, slightdifferences were observed in the metabolomes and proteomes of smoke water andGR24-treated seedlings, both with and without the addition of salt stress. Relativelyfew of the differentially expressed proteins could be identified with the instrumentsavailable. Changes in the metabolome indicated that photoassimilation andphotosynthesis could be affected in response to smoke water and GR24 treatment.Our results suggest that smoke water and GR24 both promote growth under saltstress conditions in seedlings and we furthermore conclude that, although there aredistinct overlaps between treatments, this is accomplished via slightly differentmechanisms.
[发布日期]  [发布机构] Stellenbosch University
[效力级别]  [学科分类] 
[关键词]  [时效性] 
   浏览次数:3      统一登录查看全文      激活码登录查看全文