In this study two new species of the genus Trialeurodes Cockerell from Yucatan peninsula are described based on morphological characteristics of the pupae cases, which were mounted on microscope slides and observed with a compound microscope. The new species found are: Trialeurodes quintanarroensis sp. nov. on Gymnopodium floribundum Rofle in Tulum, State of Quintana Roo; T. campecheanensis sp. nov. on Piscidia piscipula L. in Champoton and Campeche, in the state of Campeche.