The employment of parasitoidmicrowasps of pupae state of Musca domestica L. emerge as an importantalternative of biological control which could result effective. In the currentresearch, a comparative study of the parasitical activity of Spalangia endiusWalker and Muscidifurax raptorellus Kogan & Legner over pupae ofM. domestica in laboratory bioassays was performed, under controlledcondition of temperature (according to each assay), relative humidity (75 ±3 %) and darkness. The temperature (15°C, 20°C, 25 °C and 28 °C)influence on biological cycle and longevity of both parasitoids was analyzed,being 25 °C, the optimal temperature of breeding. At this temperature, thebiological cycle duration of S. endius (22.6 days) was greater in comparisonto M. raptorellus (14.8 days). The biological cycle length was in inverserelation with temperature for both species. The longevity in both species wasstatistically the same for both species (20.7 days for S. endius and18.6 days for M. raptorellus). The oviposition capacity at 25 °Cwith 20 pupae as constant host population was analyzed, obtaining for S.endius, 15 days of oviposition with 175 parasitized pupae in total; by contrastM. raptorellus parasitized 140 pupae in 16 days. The highest ovipositionfor both species was the third day. The phenological state of maturation oversusceptible pupae of M. domestica to be parasitized was determined, at25 °C in groups of 50 pupae of 1, 2, 3 and 4 days old. The pupae of twodays of maturation were the most parasitized obtaining 66.4 % of effective parasitismfor S. endius and 60.2 % for M.raptorellus Moreover, the optimal number of pupae of housefly to be exposeto both parasitoid species was determined; of the nine relations of parasitismperformed (1:5 to 1:50), the optimal number of pupae was the relation 1:10.Finally, in laboratory conditions, the microwasp S. endius resultedbeing the parasitoid more effective in comparison to M. raptorellus andcould be considerate an efficient biological control of pupae of M. domestica.
