We report horse-flies collections with Malaise traps from the medium rainforest of Yucatan during 2007. We recorded eight species of Tabanidae (Chrysops variegatus, Diachlorus ferrugatus, Leucotabanus canithorax, L. itzarum, Tabanus colombensis, T. commixtus, T. haemagogus and T. oculus) which represented 36% of known species for this state. Tabaninae was the subfamily best represented (seven species and three genera). The most abundant species were Tabanus commixtus and Leucotabanus itzarum.