Virulence of luminous vibrios to Artemiafranciscana nauplii

[摘要] ABSTRACT: From healthy and diseased penaeid shrimp from Asia and the Americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as Vibrio harveyi; 9 isolates produced significantmortalities (45 to 80%) in Artemia franciscana nauplii at inoculation densities of 10⁵ to 10⁶ CFU ml^-1 compared to the controls (unchallenged nauplii). The maximum number of bacteria ingested (bioencapsulated) bythe Artemia nauplii varied from less than 10 to 10³ CFU nauplius^-1 and no significant relationship was observed between the density of bacteria inoculated, the amount of bacteria ingested, and naupliar mortality. Significantcorrelations were obtained between naupliar mortality and production of proteases, phospholipases or siderophores, but not between mortality and lipase production, gelatinase production, hydrophobicity or hemolytic activity. The results suggest thatvirulence of the strains tested was more related to the production of particular exoenzymes than to the measured colonization factors.

[学科分类] 生物科学（综合）

[关键词] Pathogenicity;Luminous Vibrio;Hydrophobicity;Exoenzymes;Artemia