Efficacy of various chemotherapeutic agents on the growth of Spironucleus vortens, an intestinal parasite of the freshwater angelfish
[摘要] ABSTRACT: Seven chemotherapeutic agents (dimetridazole, metronidazole, pyrimethamine, albendazole, fenbendazole, mebendazole and magnesium sulfate) were examined for growth inhibition on the cultivation of
Spironucleus vortens. Dimetridazoleand metronidazole were effective in inhibiting the parasite's growth. At concentrations of 1 µg ml
-1 or higher, both dramatically decreased numbers of parasites. At 24 h exposure, 33% of parasites were inhibited when exposed to dimetridazoleor metronidazole at concentrations of 2 and 4 µg ml
-1, respectively. Dimetridazole at 4 µg ml
-1 or higher concentrations decreased the number of organisms to 50% or less after 48 h exposure. During the same period of time, thenumbers of parasites decreased to 50% or less when exposed to metronidazole at 6 µg ml
-1 or higher. Pyrimethamine at concentrations of 1 to 10 µg ml
-1 was not effective in inhibiting the parasite's growth. Albendazole andfenbendazole at concentrations of 0.1 and 0.5 µg ml
-1 were similar in inhibiting the growth of the organism. Both compounds suppressed parasite growth at concentrations of 1.0 µg ml
-1 or higher after 24 h exposure. Mebendazoleinhibited the parasite's growth at concentrations of 0.5 µg ml
-1 or higher. At 72 h exposure, 45 to 50% of the parasites were inhibited when exposed to mebendazole at concentrations higher than 0.5 µg ml
-1. Magnesium sulfate atconcentrations of 70 mg ml
-1 or higher also suppressed the growth of parasites after 24 h exposure. These results indicate that dimetridazole, metronidazole and mebendazole are the most effective chemotherapeutic agents
in vitroat inhibiting the growth of
S. vortens.
