Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae

[摘要] ABSTRACT: We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 × 10⁴ TCID₅₀ (50% tissue culture infectious dose) ml^–1. Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 × 10⁷ virus particles ml^–1 seawater.

[学科分类] 生物科学（综合）

[关键词] Nodavirus;RT-PCR;Real-time PCR;Quantification;Seawater;Atlantic halibut