Detection of infectious salmon anemia virus insea water by nested RT-PCR

[摘要] ABSTRACT: A method to detect low levels of infectious salmon anemia virus (ISAV) in environmental samples has been developed. The method is based on concentrating the viruses by tangential flow filtration and ultracentrifugation prior to amplification ofthe extracted viral RNA by nested reverse transcription polymerase chain reaction (RT-PCR). In the current investigation, seawater samples from salmon holdings were used for ISAV identification. ISAV was detected in seawater samples from a salmon holdingsite and from a vessel transporting salmon in 2 consecutive trials of the methodology. When known concentrations of ISAV were added to 2 l of sea water, 5.5 viruses ml^-1 (corresponding to a tissue culture titer of TCID₅₀ 1.6× 10^-2 ml^-1) were detected using this method.

[学科分类] 生物科学（综合）

[关键词] Infectious salmon anemia virus;Nested RT-PCR;Virus concentration;Salmo salar