Low sequence variation among isolates of infectious hypodermal and hematopoieticnecrosis virus (IHHNV) originating fromHawaii and the Americas

[摘要] ABSTRACT: A 2.9 kb fragment of the infectious hypodermal and hematopoietic necrosis virus (IHHNV) genome, which contains the coding sequence of putative non-structural and capsid proteins, was amplified and sequenced from each of 14 IHHNV isolatescollected from cultured penaeid shrimp stocks in Hawaii and various sites in the Americas between 1982 and 1997. The sequence comparison indicates that the IHHNV genome is very stable, with 99.6 to 100% similarity among these 14 isolates. Only nucleotidesubstitutions were found. The percentage of substitution was higher in the putative capsid proteins region (1.3%) than in the putative non-structural proteins region (0.6%). Out of 25 substitutions found, 14 resulted in amino acid changes. There is noapparent association between clinical outcomes and particular amino acid substitutions. Based on genetic distances, the isolates were clustered into 3 groups that generally correspond with their geographic origins.

[学科分类] 生物科学（综合）

[关键词] Shrimp virus;Infectious hypodermal and hematopoietic necrosis virus;IHHNV;Sequence variation