Identification of Somatotrophs, Mammosomatotrophs, and Mammotrophs by Sandwich Cell Immunoblot Assay in GH- Secreting Adenomas and Prolactinomas: Correlations between the Proportions of Hormone Secreting Cells and Tumor Size

[摘要] References(38)Cited-By(3)Sandwich cell Immunoblot assay(sandwich CIBA) was used to identify somatotrophs (GH cells), mammosomatotrophs, and mammotrophs (PRL cells) in pituitary tumors obtained from patients with GH-secreting adenomas and prolactinomas. The mean serum GH level was 177.6ng/ml in 19 patients with GH-secreting adenomas and the mean PRL level was 2, 129ng/ml in 9 patients with prolactinomas. GH-secreting adenomas could be divided into 3 groups according to the proportions of the cell types. The GH cell dominant type had more than 70% GH cells. The mammosomatotroph cell dominant type had more than 80% mammosomatotrophs. The nondominant type had no dominant cell type. There was a good correlation (r2=0.804) between serum GH levels and tumor size in patients with the GH cell dominant type (n=10). The nondominant type (n=8) had a low serum level of GH except for one tumor. The mammosomatotroph cell dominant type (n=1) showed high serum levels of both GH and PRL. All prolactinomas had a predominance of PRL cells. Sandwich CIBA is a simple method for detecting GH cells, mammosomatotrophs, and PRL cells, and useful for classification for GH-secreting adenomas.

[学科分类] 内分泌与代谢学

[关键词] Pituitary adenoma;GH-secreting adenoma;Prolactinoma;Sandwich cell immunoblot assay